TY  - BOOK
AU  - Riobo,Natalia A.
TI  - Hedgehog signaling protocols
T2  - Methods in molecular biology,
SN  - 9781493927715
AV  - QP517.C45 H44 2015
U1  - 571.7/4 23
PY  - 2015///]
CY  - New York
PB  - Humana Press
KW  - Hedgehog Proteins
KW  - Research
KW  - Methodology
KW  - Cellular signal transduction
KW  - Transcription factors
KW  - Signal Transduction
KW  - Transcription Factors
KW  - Hedgehog proteins
KW  - Laboratory Manuals
N1  - Includes bibliographical references and index; Luciferase reporter assays to study transcriptional activity of hedgehog signaling in normal and cancer cells; Silvia Pandolfi and Barbara Stecca --; Measuring expression levels of endogenous gli genes by immunoblotting and real-time PCR; Pawel Niewiadomski and Rajat Rohatgi --; Quantitative immunoblotting of endogenous hedgehog pathway components; Shohreh F. Farzan and David J. Robbins --; Measuring Gli2 phosphorylation by selected reaction monitoring mass spectrometry; Robert Ahrends, Pawel Niewiadomski, Mary N. Teruel, and Rajat Rohatgi --; Rapid screening of Gli2/3 mutants using the Flp-In system; Pawel Niewiadomski and Rajat Rohatgi --; Insights into gli factors ubiquitylation methods; Paola Infante, Romina Alfonsi, and Lucia Di Marcotullio --; Determination of acetylation of the gli transcription factors; Sonia Coni, Laura Di Magno, and Gianluca Canettieri; Efficient detection of indian hedgehog during endochondral ossification by whole-mount immunofluorescence; João Francisco Botelho, Daniel Smith-Paredes, and Veronica Palma A. --; Methods for detection of Ptc1-driven LacZ expression in adult mouse skin; Donna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo --; Determination and analysis of cellular metabolic changes by noncanonical hedgehog signaling; Raffaele Teperino and John Andrew Pospisilik
UR  - http://catdir.loc.gov/catdir/enhancements/fy1512/2015942639-t.html
UR  - https://www.loc.gov/catdir/enhancements/fy1618/2015942639-d.html
ER  -