Hedgehog signaling protocols / edited by Natalia A. Riobo, Department of Biochemistry and Molecular Biology. Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA.
Material type:
TextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1322. | Springer protocols (Series)Publisher: New York : Humana Press, [2015]Edition: Second editionDescription: xvi, 200 pages : illustrations (some color) ; 26 cmContent type: text Media type: unmediated Carrier type: volumeISBN: 9781493927715; 149392771XSubject(s): Hedgehog Proteins -- Research -- Methodology | Cellular signal transduction -- Research -- Methodology | Transcription factors -- Research -- Methodology | Signal Transduction | Transcription Factors | Hedgehog proteinsGenre/Form: Laboratory Manuals.DDC classification: 571.7/4 LOC classification: QP517.C45 | H44 2015NLM classification: W1 ME9616J | QU 55.2Online resources: Table of contents | Publisher description | Item type | Current library | Call number | Copy number | Status | Date due | Barcode |
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KsTU Library General Stacks | QP517.C45 H44 2015 (Browse shelf(Opens below)) | 1 | Available | 102500 |
Includes bibliographical references and index.
Luciferase reporter assays to study transcriptional activity of hedgehog signaling in normal and cancer cells / Silvia Pandolfi and Barbara Stecca -- Measuring expression levels of endogenous gli genes by immunoblotting and real-time PCR / Pawel Niewiadomski and Rajat Rohatgi -- Quantitative immunoblotting of endogenous hedgehog pathway components / Shohreh F. Farzan and David J. Robbins -- Measuring Gli2 phosphorylation by selected reaction monitoring mass spectrometry / Robert Ahrends, Pawel Niewiadomski, Mary N. Teruel, and Rajat Rohatgi -- Rapid screening of Gli2/3 mutants using the Flp-In system / Pawel Niewiadomski and Rajat Rohatgi -- Insights into gli factors ubiquitylation methods / Paola Infante, Romina Alfonsi, and Lucia Di Marcotullio -- Determination of acetylation of the gli transcription factors / Sonia Coni, Laura Di Magno, and Gianluca Canettieri.
Efficient detection of indian hedgehog during endochondral ossification by whole-mount immunofluorescence / João Francisco Botelho, Daniel Smith-Paredes, and Veronica Palma A. -- Methods for detection of Ptc1-driven LacZ expression in adult mouse skin / Donna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo -- Determination and analysis of cellular metabolic changes by noncanonical hedgehog signaling / Raffaele Teperino and John Andrew Pospisilik.
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