000			03607cam a22005657i 4500
001			18635724
003			OSt
005			20220623151118.0
008			150528s2015 nyua b 001 0 eng d
010			_a 2015942639
016	7		_a101667763 _2DNLM
020			_a9781493927715 _q(hbk : alk. paper)
020			_a149392771X _q(hbk : alk. paper)
020			_z9781493927722 _q(eBook)
020			_z1493927728 _q(eBook)
035			_a(OCoLC)ocn905638802
040			_aYDXCP _beng _cNLM _erda _dYDXCP _dIDL _dNUI _dIAL _dOCLCO _dOCLCQ _dOCLCO _dDLC
042			_anlmcopyc _alccopycat
050	0	0	_aQP517.C45 _bH44 2015
060	0	0	_aW1 ME9616J _bobr 2015
060	1	0	_aQU 55.2
082	0	0	_a571.7/4 _223
245	0	0	_aHedgehog signaling protocols / _cedited by Natalia A. Riobo, Department of Biochemistry and Molecular Biology. Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA.
250			_aSecond edition.
264		1	_aNew York : _bHumana Press, _c[2015]
300			_axvi, 200 pages : _billustrations (some color) ; _c26 cm.
336			_atext _btxt _2rdacontent
337			_aunmediated _bn _2rdamedia
338			_avolume _bnc _2rdacarrier
490	1		_aMethods in molecular biology, _x1064-3745 ; _v1322
490	1		_aSpringer protocols
504			_aIncludes bibliographical references and index.
505	8	0	_tLuciferase reporter assays to study transcriptional activity of hedgehog signaling in normal and cancer cells / _rSilvia Pandolfi and Barbara Stecca -- _tMeasuring expression levels of endogenous gli genes by immunoblotting and real-time PCR / _rPawel Niewiadomski and Rajat Rohatgi -- _tQuantitative immunoblotting of endogenous hedgehog pathway components / _rShohreh F. Farzan and David J. Robbins -- _tMeasuring Gli2 phosphorylation by selected reaction monitoring mass spectrometry / _rRobert Ahrends, Pawel Niewiadomski, Mary N. Teruel, and Rajat Rohatgi -- _tRapid screening of Gli2/3 mutants using the Flp-In system / _rPawel Niewiadomski and Rajat Rohatgi -- _tInsights into gli factors ubiquitylation methods / _rPaola Infante, Romina Alfonsi, and Lucia Di Marcotullio -- _tDetermination of acetylation of the gli transcription factors / _rSonia Coni, Laura Di Magno, and Gianluca Canettieri.
505	8	0	_tEfficient detection of indian hedgehog during endochondral ossification by whole-mount immunofluorescence / _rJoão Francisco Botelho, Daniel Smith-Paredes, and Veronica Palma A. -- _tMethods for detection of Ptc1-driven LacZ expression in adult mouse skin / _rDonna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo -- _tDetermination and analysis of cellular metabolic changes by noncanonical hedgehog signaling / _rRaffaele Teperino and John Andrew Pospisilik.
650	1	2	_aHedgehog Proteins _xResearch _xMethodology.
650		0	_aCellular signal transduction _xResearch _xMethodology.
650		0	_aTranscription factors _xResearch _xMethodology.
650	2	2	_aSignal Transduction.
650	2	2	_aTranscription Factors.
650		0	_aHedgehog proteins.
655		2	_aLaboratory Manuals.
700	1		_aRiobo, Natalia A., _eeditor.
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 1322. _x1064-3745
830		0	_aSpringer protocols (Series) _x1949-2448
856	4	1	_3Table of contents _uhttp://catdir.loc.gov/catdir/enhancements/fy1512/2015942639-t.html
856	4	2	_3Publisher description _uhttps://www.loc.gov/catdir/enhancements/fy1618/2015942639-d.html
906			_a7 _bcbc _ccopycat _d2 _encip _f20 _gy-gencatlg
942			_2lcc _cBK _n0
999			_c954 _d954